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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Cancer Immunol Res. 2013 Aug;1:99–111. doi: 10.1158/2326-6066.CIR-13-0047

Figure 6.

Figure 6

TRP1 CD8 T cells delay tumor growth in vivo. A) Naïve CD8 T cells were harvested from TRP1high, TRP1low or wild type mice and transferred at 3 million cells per mouse into female C57BL/6 recipients. On the same day, 50,000 B16 tumor cells were inoculated subcutaneously. Tumor volume was measured over time, and mice were euthanized when tumor size reached 2 cm3. n=30 mice per group, pooled from 3 independent experiments. **p=0.0001 vs. polyclonal CD8. B) CD8 T cells were harvested from TRP1 TN mice and cultured with BMDCs pulsed with TRP1 heteroclytic peptide for 18 hours. CD8 cells were harvested, washed, counted and transferred to recipient C57BL/6 mice at 3 million cells per mouse one day after inoculation with 50,000 B16 cells. n=30 mice per group, pooled from 3 independent experiments. *p=0.0119, **p=0.0161 vs. polyclonal CD8. C) CD8 T cells were harvested from TRP1low or C57BL/6 donor mice and transferred into recipients either directly (naïve) or after 18 hours of culture with peptide-pulsed BMDCs (activated). Recipient mice were inoculated with B16 cells on the same day as naïve cell transfer, or one day prior to activated cell transfer. **p=0.001 vs polyclonal CD8 naïve, ***p=0.0005 vs, polyclonal CD8 activated. D) CD8 T cells were harvested from TRP1 TN mice and cultured with BMDCs pulsed with TRP1 heteroclytic peptide for 18 hours. CD8 cells were harvested, washed, counted and transferred to recipient C57BL/6 mice at the indicated number of cells per mouse one day after inoculation with 50,000 B16 cells. n=10 mice per group. *p=0.0361, **p=0.012, ***p=.0045, ****p=0.0011, *****p<0.0001 vs. polyclonal CD8. E) CD8 T cells were harvested from TRP1 TN mice and cultured with BMDCs pulsed with TRP1 heteroclytic peptide for 18 hours. CD8 cells were harvested, washed, counted and transferred to recipient C57BL/6 mice at 300,000 cells per mouse one day after inoculation with 50,000 B16 cells. n=10 mice per group. *p=0.0047, **p=0.0008, *** p=0.0004 vs. polyclonal CD8. F) CD8 T cells were harvested from C57BL/6 mice or TRP1high mice and cultured for 5 days with anti-CD3/CD28 coated beads. CD8 cells were harvested, washed, counted and transferred into C57BL/6 recipient mice at 300,000 cells per mouse one day after inoculation with 50,000 B16 cells. **p=0.0021.