Skip to main content
. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Mol Cell Neurosci. 2013 Oct 16;57:111–119. doi: 10.1016/j.mcn.2013.10.006

FIGURE 1. HspB1 reverses the inhibitory effect of Nogo-A on neurite growth.

FIGURE 1

A. vHspB1 construct. B. Cortical neurons transfected with vHspB1 express HspB1 mRNA (left) and protein (right). C. HspB1 is expressed in the majority of neurons in the culture after transfection with vHspB1. D. Transgene mediated HspB1 (green) was found in neurites and growth cones (arrows) where HspB1 co-localized with phalloidin binding F-actin (red). Scale bar = 10 µm. E. Transfection with vHspB1 prevented inhibition of neurite extension by NogoA-Fc (right panels). Immunostained with anti-βIII tubulin antibody; scale bar = 20µm. F. Quantitative analysis of neurite length. (n=3; **P < 0.01).