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. 1971 Dec;68(12):3122–3126. doi: 10.1073/pnas.68.12.3122

A Complex Between Initiation Factor IF2, Guanosine Triphosphate, and fMet-tRNAf: An Intermediate in Initiation Complex Formation

Arthur H Lockwood 1, Prasanta R Chakraborty 1, Umadas Maitra 1
PMCID: PMC389604  PMID: 4943554

Abstract

Evidence is presented that suggests the formation of a complex between polypeptide chain-initiation factor IF 2, GTP, and fMet-tRNAf. This complex transfers both fMet-tRNAf and GTP to 30S ribosomal subunits in the presence of ApUpG and initiation factor IF 1. The resultant 30S initiation complex reacts with 50S subunits to form a 70S initiation complex. fMet-tRNAf in this 70S complex reacts with puromycin to form fMet-puromycin. These results suggest that [IF 2, GTP, fMet-tRNAf] is an intermediate in the initiation of protein synthesis in Escherichia coli.

Keywords: E. coli, protein biosynthesis, 30S ribosomal subunits, Sephadex filtration, 70S ribosomal complex

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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