(A, B) Annexin V and PI (not shown) positivity was measured in asymptomatic or poly-symptomatic VL dogs after no stimulation and stimulation with 10 µg/ml f-t L.i. for 7 days. PBMC were gated on CD4+ (A) or CD8+ (B) live lymphocytes based on isotype control. (C, D) Annexin V positivity was measured similarly to 3A, after blocking with isotype, IL-10, or B7.H1 blocking antibodies in CD4+ (C) and CD8+ (D). **p<0.01 via one way ANOVA with Tukey’s post-test in GraphPad Prism 5. Experiments included n=10.