(A) NBT assay performed on adherent PBMC from asymptomatic and symptomatic VL dogs after isotype, B7.H1, or IL-10 antibody treatment and stimulation with f-t L.i.. (B)
L. infantum-specific quantitative RT-PCR performed on PBMC DNA. Data presented as number of genomic copies of L. infantum/106 PBMC from symptomatic dogs, after f-t L.i. stimulation (Li) compared to stimulation and addition of isotype, (left, Iso), anti-IL-10 (middle, IL-10), or anti-B7.H1antibody (right, B7.H1). (A) n=13 and (B) n=7. *p<0.05, **p<0.01, via one way ANOVA with Tukey’s post-test on averaged (A) or log-transformed (B) data.