Figure 5.

The effect of X-ASA analogue, pBr-ASA, and pNO-ASA on DAF fluorescence in HepG2 cells. HepG2 cells were treated with DMSO, pNO-ASA (25 μM) and pBr-ASA (25 μM) for 24 h or spermine NONOate (25 μM) for 20 min. After treatment cells were labeled with DAF-DA and Hoechst nuclear stain. In similar experiments, the effect of NOS inhibition on pBr-ASA treatment showed no effect relative to pBr-ASA alone (Figure S1 in the Supporting Information).