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LC-MS/MS analysis of GST-P1 modification by QM at Cys47 and Cys101. GST-P1 (15 μM) was treated with pNO-ASA or pBr-ASA (30 μM) for 30 min and unreacted Cys blocked with NEM (20 mM). The protein after in-gel digest was analyzed by LC-MS/MS, yielding extracted ion chromatograms of: a) QM modified Cys47 peptide, and; b) QM modified Cys101 peptide. The MS/MS spectra with b and y ion fragmentations are shown for: c) QM modified Cys47 peptide, and; d) QM modified Cys101 peptide.
