Table 1.
Identified peptide fragment (starting-ending residue) | Cys | % Modification | |
---|---|---|---|
QM | NEM | ||
FLPLQSQCPEGAGDAVMYASTECK (16-39) | Cys23 | 19 | 81 |
Cys38 | - | 100 | |
LSQQLCDVTLQVK (72-84) | Cys77 | - | 100 |
CVLHVMNGAVMYQIDSVVR (151-169) | Cys151 | - | 100 |
ACSDFLVQQLDPSNAIGIANFAEQIGCVELHQR (170-202) | Cys171 | - | 100 |
Cys196 | 14 | 86 | |
QEEFFNLSHCQLVTLISR (217-234) | Cys226 | 28 | 72 |
CESEVFHACINWVK (241-254) | Cys241 | - | 100 |
Cys249 | 14 | 86 | |
CHSLTPNFLQMQLQK (273-287) | Cys273 | 8 | 92 |
IFEELTLHKPTQVMPCR (304-320) | Cys319 | 10 | 90 |
SGLAGCVVGGLLYAVGGR (363-380) | Cys368 | - | 100 |
NNSPDGNTDSSALDCYNPMTNQWSPCAPMS VPR (381-413) | Cys395 | - | 100 |
Cys406 | - | 100 | |
IGVGVIDGHIYAVGGSHGCIHHNSVER (416-442) | Cys434 | - | 100 |
SGAGVCVLHNCIYAAGGYDGQDQLNSVER (508-536) | Cys513 | - | 100 |
Cys518 | - | 100 | |
IYVLGGYDGHTFLDSVECYDPDTDTWSEVTR (566-596) | Cys583 | - | 100 |
SGVGVAVTMEPCR (602-614) | Cys613 | - | 100 |
Keap1 (15 μM) was incubated with pNO-ASA (30 μM) for 30 minutes followed by addition of NEM (20 mM) to trap unreacted free thiols.