The prevalences of NASH based on the presence of four risk factors found to be independently predictive of NASH (PNPLA3 G, CRP > 0.80 mg/dl, glucose > 100 mg/dl, and CK-18 > 145IU/l). In patients with no risk factor the probability of NASH was 9% (95% confidence intervals: 3–24%), whereas if all four risk factors were present the probability of NASH increased to 82% (65–91%). Only three patients in our study had no risk factor, 32 had one risk factor, 45 had two risk factors, 44 had three risk factors, and nine patients had all four risk factors.