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. Author manuscript; available in PMC: 2014 Apr 10.
Published in final edited form as: Nature. 2013 Oct 2;502(7470):194–200. doi: 10.1038/nature12639

Figure 2. Hedgehog signaling requires the primary cilia to regulate autophagy.

Figure 2

(a) GFP-LC3 in MEFs with Purmorphamine (Purmo; *p=0.028, n=4), Ptc−/− (*p=0.015, n=4) and myc-Gli1 overexpression (*p=0.012, n=3). (b) mCherry-GFP-LC3 upon Purmo treatment in MEFs. Yellow, autophagosomes; red, autophagolysosomes; total, both. (*p=0.0323, 40 fields). (c) LC3 flux in Ctrl (**p=0.001) and Smo(−) MEFs (**p=0.0001) upon Purmo (*p=0.009). (n=4). (d) mCherry-GFP-LC3 in wt and IFT88−/− KECs upon Purmo. (wt Purmo, #p=0.046; wt Serum-, #p=0.008, 40 fields). (e) LC3 flux in IFT88−/− KECs overexpressing myc-Gli1 (**p=0.00006, n=3). (f) mCherry-GFP-LC3 in MEFs with Cyclopamine (Cyclo). (*p=0.011, #p=0.013, 25 fields). Bars: 10µm. n.s., statistically non-significant. Mean±s.d in c, f and mean±s.e.m rest.