Table 1.
Saccharification data of wood from both field trials
| Line | Pretreatment | Saccharification yield, % dry weight | Increase in saccharification yield compared to WT, % | Cellulose conversion, % cellulose | Increase in cellulose conversion compared to WT, % |
| Belgian trial | |||||
| FS3 | None | 10.5 ± 2.5 | 14.1 | 23.3 ± 3.9 | 39.5 |
| 0.4 M H2SO4 | 26.5 ± 4.3 | 18.8 | 55.7 ± 5.2 | 28.3 | |
| 6.25 mM NaOH | 32.0 ± 5.4 | 14.7 | 67.6 ± 7.0 | 31.8 | |
| 62.5 mM NaOH | 39.3 ± 4.7 | 11.3 | 79.3 ± 6.8 | 15.1 | |
| FS40 | None | 12.8 ± 2.3 | 39.1 | 25.4 ± 4.6 | 52.1 |
| 0.4 M H2SO4 | 28.3 ± 3.2 | 26.9 | 56.7 ± 5.0 | 30.6 | |
| 6.25 mM NaOH | 33.8 ± 4.9 | 21.1 | 68.7 ± 9.7 | 33.9 | |
| 62.5 mM NaOH | 39.3 ± 4.8 | 11.3 | 79.7 ± 9.3 | 15.7 | |
| WT | None | 9.2 ± 1.2 | 16.7 ± 1.7 | ||
| 0.4 M H2SO4 | 22.3 ± 3.5 | 43.4 ± 4.5 | |||
| 6.25 mM NaOH | 27.9 ± 3.3 | 51.3 ± 4.1 | |||
| 62.5 mM NaOH | 35.3 ± 2.6 | 68.9 ± 4.0 | |||
| French trial | |||||
| FS3 | None | 9.9 ± 2.2 | 25.3 | 19.9 ± 7.2 | 17.1 |
| 0.4 M H2SO4 | 18.1 ± 3.5 | 33.1 | 36.2 ± 10.4 | 23.5 | |
| 6.25 mM NaOH | 23.6 ± 3.1 | 24.9 | 46.4 ± 12.4 | 14.3 | |
| 62.5 mM NaOH | 28.6 ± 3.3 | 14.9 | 56.4 ± 14.5 | 5.6 | |
| FAS13 | None | 18.9 ± 3.8 | 139.2 | 34.7 ± 8.2 | 104.1 |
| 0.4 M H2SO4 | 26.5 ± 2.9 | 94.9 | 48.3 ± 5.7 | 64.8 | |
| 6.25 mM NaOH | 33.7 ± 2.6 | 78.3 | 60.6 ± 5.6 | 49.3 | |
| 62.5 mM NaOH | 36.2 ± 2.1 | 45.4 | 65.2 ± 5.4 | 22.1 | |
| WT | None | 7.9 ± 1.9 | 17.0 ± 5.2 | ||
| 0.4 M H2SO4 | 13.6 ± 2.1 | 29.3 ± 6.8 | |||
| 6.25 mM NaOH | 18.9 ± 2.6 | 40.6 ± 10.1 | |||
| 62.5 mM NaOH | 24.9 ± 2.3 | 53.4 ± 12.0 |
Saccharification yields are the mean (± SD) for all trees of redness class 5 for the Belgian field trial whereas cellulose measurements were performed on selected individual trees only (Table S1) (WT, n = 6; FS3, n = 6; FS40, n = 7). To calculate cellulose conversions, the specific saccharification yields of those individual trees for which cellulose was measured were used. For each of the five blocks in the French field trial, saccharification yield was determined for the five most red trees per transgenic line and five randomly chosen WT trees. For the French field trial, cellulose conversion was determined for pooled trees (Table S1) (WT, n = 10; FS3, n = 10; FAS13, n = 10). The cell-wall residue (expressed as % dry weight) was taken into account to calculate the cellulose conversions. Bold, significantly increased compared with WT.