Fig. 1.
FLIP proteins inhibit IFN-β enhancer activation independent of NF-κB. Subconfluent (A) wild-type or (B) p65−/− MEF cellular monolayers were cotransfected with pIFN-β–luc (225 ng), pRL-null (25 ng), 500 ng pMAVS or pCI, and 750 ng of pCI, pMC159, pMC160, pcFLIPL, pK13, or pnsp11. Values are shown as mean ± SD (n = 3). Twenty-four hours later, cells were lysed and firefly and sea pansy luciferase activities were measured. A portion of each lysate was analyzed for FLIP expression by immunoblotting, using the antiserum indicated. These are representative results from experiments that were performed at least three times independently.