Fig. 2.

I-astrocytes express prototypic astrocyte markers. (A) Immunofluorescence analysis reveals strong up-regulation of astrocytic markers such as Vimentin, CD44, S100β, and GFAP in i-astrocytes compared with the initial fibroblasts. DAPI (blue) was used to visualize nuclei. (B) RT-PCR analysis revealed expression of IGFB3 in fibroblasts, iNPCs, and i-astrocytes, whereas expression of S100β and Aqp4 was detected in iNPCs and i-astrocytes or i-astrocytes only, respectively. (Scale bar: 100 μm.) Fibro, fibroblast. i-Astro, i-astrocytes.