Fig. 3.

I-astrocytes from fALS and sALS patients display toxicity toward MNs. (A) Representative images after 96 h of coculture of HB9-GFP expressing MNs (shown in black) with astrocytes from spinal cord (sc) or skin of ALS patients and controls. A marked loss of MN viability was observed in the presence of ALS astrocytes irrespective of their origin (spinal cord or skin). (B) Relative percentage of MN survival after 96 h of coculture with ALS astrocytes derived from spinal cord or skin and their respective controls. ***P < 0.001; ****P < 0.0001 [compared with the average taken from of all converted control lines (HDFA, 8620, 155, 170)]. Error bars represent SEM. Quantification was performed in triplicate wells of a 96-well plate, and data are representative of n = 5.