Figure 3. Murine HSCs express high levels of EmGFP/IL-15.

(A) HSCs were identified in the BM of reporter mice and littermate controls as Lin^-Sca-1⁺c-kit⁺ (LSK). Data are representative of at least 2 independent experiments. Lin^- = CD3^-CD19^-CD11b^-CD11c^-Gr-1^-NK1.1^-Ter119^-. (B-C) Thymocytes from reporter mice and littermate controls were examined for expression of EmGFP/IL-15. Double negative (DN) thymocyte populations were defined as follows: CD3^-CD4^-CD8-; ETP, c-kit⁺CD25^-; DN2, CD44^highCD25⁺, both c-kit⁺ and c-kit^-; DN3, CD44^-CD25⁺; DN4, CD44^-CD25^-. Double positive (DP) cells were CD3^-CD4⁺CD8⁺, and single positive (SP) cells were either CD3⁺CD4^-CD8⁺ or CD3⁺CD4⁺CD8^-. The MFI of EmGFP/IL-15 is shown graphically for each population in B. Tg^- (gray histograms), Tg⁺ (black line).