Skip to main content
. 2014 Jan 20;9(1):e85037. doi: 10.1371/journal.pone.0085037

Figure 1. Adipokine secretion in the trans-well versus contact co-culture system.

Figure 1

Adipokine secretion after the 12-culture incubation in the contact (A) and trans-well (B) treated co-culture conditions, and (C) a comparison of secreted MCP1, IL-6 and TNFα between the contact and trans-well co-culture systems. Note: no IL-10 was detected in the trans-well system (B). 0 hr  =  serum starved adipocytes alone prior to co-culture and fatty acid treatment, (−)  =  negative control; adipocytes alone treated with 25 µM BSA, (+)  =  positive control; co-cultured adipocytes and macrophages plus 25 µM BSA, DHA =  co-cultured adipocytes and macrophages in the presence of 125 µM DHA, EPA =  co-cultured adipocytes and macrophages in the presence of 125 µM EPA, and PA =  co-cultured adipocytes and macrophages in the presence of 125 µM PA. Values are means ± SEM. The experiment was independently conducted 3 times for a final sample size of n =  6–9. A different letter or an asterisk (*) indicates treatments are significantly different from each other, p≤0.05.