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. 2014 Jan 20;9(1):e85037. doi: 10.1371/journal.pone.0085037

Figure 4. M1 and M2 macrophage polarization marker mRNA expression in trans-well co-cultured macrophages.

Figure 4

mRNA expression of key (A) M1 (Nos2, Nfκb and Tnfα), (B) M2 (Arg1, Mrc2) polarization genes, and (C) regulatory cytokines (Tgfβ1 and Il-10) from macrophages harvested from the trans-well system at 12 hr. 0 hr  =  serum starved adipocytes alone prior to co-culture and fatty acid treatment, (+)  =  positive control; co-cultured adipocytes and macrophages plus 25 µM BSA, DHA  =  co-cultured adipocytes and macrophages in the presence of 125 µM DHA, EPA =  co-cultured adipocytes and macrophages in the presence of 125 µM EPA, and PA =  co-cultured adipocytes and macrophages in the presence of 125 µM PA. Values are mean fold change ± SEM. The experiment was independently conducted 2 times (in triplicate) for a final sample size of n = 6. A different letter indicates treatments are significantly different from each other, p≤0.05.