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. 2014 Jan 20;9(1):e85085. doi: 10.1371/journal.pone.0085085

Figure 1. IL-4 induces ALOX15 expression in A549 cells.

Figure 1

A549 cells were cultured in RPMI 1640 medium containing 50/mL IL-4 for indicated time points. (A) Total RNA was purified and ALOX15 and UTX mRNA was measured by qRT-PCR; (B) and the protein level of ALOX15 was measured by Western blot; (C) the dose effects of IL-4 on ALOX15 induction was determined by qRT-PCR. The western blot results represent two independent experiments, and the error bars represent standard error mean of three independent qRT-PCR experiments.