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. 2014 Jan 20;9(1):e85089. doi: 10.1371/journal.pone.0085089

Figure 3. Characterization of naïve-like piPSCs produced by DOX-inducible system.

Figure 3

(A) AP staining of naïve-like piPSCs, scale bar = 50 µm. (B) Pluripotency of naïve-like piPSCs was demonstrated by immunofluorescence staining of Sox2, Nanog, SSEA1, SSEA3, SSEA4, TRA-1-60 and TRA-1-81, scale bar = 100 µm. (C) Real-time PCR analysis of expression level of pluripotency genes in pADSCs, C4-6 NpiPSCs and C4-30 NpiPSCs. (D) DNA methylation analysis of the Nanog promoter in C4-6 NpiPSCs and C4-30 NpiPSCs. (E) Karyotype analysis of naïve-like piPSCs.