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. 2014 Jan 20;9(1):e85089. doi: 10.1371/journal.pone.0085089

Figure 5. Characterization of fully reprogrammed porcine naïve-like iPSCs.

Figure 5

(A) Real-time PCR analysis of expression levels of genes associated with fully reprogramming in pADSCs, C4-6 NpiPSCs and C4-30 NpiPSCs. (B) Reverse transcription PCR analysis of differentiation markers for the three germ layers in the EBs. (C) Hematoxylin and eosin staining of naïve-like piPSCs-derived teratoma (C4-30 NpiPSCs), the tumor was differentiated into the tissues of three germ layers, including cuticulated epithelium (a, ectoderm), adipose tissue (b, mesoderm) and gut-like epithelium (c, endoderm). Black arrows and square denote the specific structure of ectoderm mesoderm and endoderm, scale bar = 100 µm.