Figure 2. “Pseudo-hypoxic” response in SDHD-ESR mouse tissues.
A. Succinate-ubiquinone oxidoreductase activity (SQR) in kidney 7 days after the start of the tamoxifen treatment. B. Relative mRNA level of Vegf, Glut1, and Phd3 genes in kidney at 7 and 21 days after the start of the tamoxifen treatment. n.d.: non-determined. C. Western blot of Hif1α with protein extracts from kidney after tamoxifen treatment. Protein extracts from the pancreas of a β-cell-specific von Hippel-Lindau gene knock-out (VHL-KO) mouse and a wild-type littermate (VHL-wt) are loaded as controls. n.a.: non-applicable. Between 3 and 8 individuals per group were analyzed in each experiment. *, P≤0.05; ***, P≤0.001.