Figure 6. Analysis of p21^WAF/Cip in SDHD-ESR-derived cell lines.

A, B. Western blot of p21^WAF1/Cip1 in total protein extracts of (A) MEFs and (B) BMK cells obtained from SDHD-ESR mice and their homozygous SdhD^{flox /+} (+/+) and heterozygous SdhD^flox/− (+/−) littermates and cultured in medium supplemented with 4-hydroxy-tamoxifen for 4 or 24 hours. Quantification of relative p21^WAF1/Cip1 band intensities in (C) MEFs and (D) BMK cells normalized to β-actin signal. Results are the average ± SEM of three independent experiments. Two different immortalized clones were generated for each genotype and cell type giving the same results. *, P≤0.05; ***, P≤0.001; for 0 hours, i.e. in the absence of 4-hydroxy tamoxifen, versus 4 or 24 hours of incubation in 4-hydroxytamoxifen.