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. 2014 Jan 20;9(1):e85755. doi: 10.1371/journal.pone.0085755

Figure 4. Modification of the endogenous BMPR1A gene by cell-permeable TALEN proteins.

Figure 4

(A) Frequency of gene disruption in HEK293 cells treated for 2 hr with 1.0 µM BMPRIA-targeting TALEN proteins conjugated at various peptide-to-protein ratios. All R9-conjugated BMPR1A-targeted TALEN proteins were labeled in the presence of protease inhibitor cocktail. (B) Comparison of the frequency of BMPR1A knockout in HEK293 cells transfected with 200 ng of TALEN expression vectors or treated with 1.0 µM cell-permeable TALEN proteins for 2 hr. Black triangles indicate expected Surveyor nuclease assay cleavage products.