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. 2014 Jan 20;9(1):e85509. doi: 10.1371/journal.pone.0085509

Figure 2. HSPB1 is cleaved by MMPs.

Figure 2

A HUVECs were treated for 24(2 µM). Cleaved HSPB1 levels in conditioned media (C.M.) were detected by western blot analysis using an HSPB1 antibody to amino acids 158-205. B Recombinant HSPB1 (2 µg/mL) was incubated with ADAMTS1, MMP3, or MMP9 (50 ng/mL) for the indicated times. HSP70 (2 µg/mL) was incubated with MMP9 as a negative control. HSPB1 cleavage was detected by immunoblot using the HSPB1 antibody to amino acids 158-205 as well as Coomassie staining. C Recombinant HSPB1 (2 µg/mL) was incubated with MMP9 (50 ng/mL) for the times indicated and analyzed by western blot using antibodies against the C- (158-205) and N-termini (10–21) of HSPB1.