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. 2014 Jan 20;9(1):e85518. doi: 10.1371/journal.pone.0085518

Figure 5. Net contains an enhancer that drives expression in both midline glia and trachea.

Figure 5

(A) A genomic region located between NetA and NetB was used to generate a reporter construct and (B) the Net enhancer is shown. (C–K) Whole mount embryos were double-stained with an anti-GFP antibody (green: D, G and J) and anti-sim (red; E) or monoclonal antibody 2A12 (red; H and K) and analyzed by confocal microscopy. The overlap in expression is shown in yellow in the merge column (C, F and I). The Net797:GFP reporter drove expression in midline glia (arrows C–E; ganglionic branches of trachea are also visible in the image in green) and occasionally midline neurons within some segments (arrowheads C–E). The tracheal expression pattern of this reporter is unique in that GFP is high in tracheal cells, except cells within the dorsal trunk (F–K). Lateral views of stage 16 transgenic embryos are shown; anterior is in the top, left hand corner and ventral is on the left.