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. 2014 Jan 20;9(1):e85518. doi: 10.1371/journal.pone.0085518

Figure 6. The comm cis-regulatory region contains an enhancer that drives expression in the embryonic midline and larval trachea.

Figure 6

(A) Genomic regions surrounding and within comm were used to generate the reporter constructs and (B) the comm enhancer is shown. The closest gene upstream of comm is CG6244, which is 79,892 bp away. (C–N) Whole mount embryos were double-stained with anti-GFP (green: D, G, J and M) and anti-sim antibodies (red; E, H, K and N) and analyzed by confocal microscopy and the overlap in expression is shown in yellow in the merge column (C, F, I and L). (C–E) comm2575:GFP and (L–N) comm443:GFP are expressed in both midline glia (arrows) and midline neurons (arrowheads), while (F–H) comm693:GFP is restricted to some midline neurons (arrowheads). The comm267:GFP (I–K) and comm737:GFP (data not shown) reporters are not expressed in the midline. Lateral views of stage 16 transgenic embryos are shown; anterior is in the top, left hand corner and ventral is on the left. (O–T) comm443:GFP is also expressed in the tracheal dorsal trunks (arrows in O and S) as well as other tracheal branches (arrow in R). Live larvae containing the comm443:GFP reporter were analyzed by confocal and differential contrast microscopy and dorsal views are shown.