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. 2014 Jan 20;9(1):e85983. doi: 10.1371/journal.pone.0085983

Figure 4. TCR repertoire analyses in Cr-induced ACD mice.

Figure 4

(A, B) TCRAV and TCRBV repertoires were analyzed from footpads of ACD and control mice by microplate hybridization assay. Spleens from corresponding Cr-injected mice and saline-injected mice were used as controls. Bars and error bars indicate mean ±SD. At 7 days after challenge, percentage frequencies of the expression levels of VA11-1, VA14-1, VB8-2, and VB14-1 were significantly higher in footpads of the ACD mice compared with the control mice (*p<0.05, unpaired Student t-test). (C) Increase in the frequencies of VA11-1, VA14-1, VB8-2, and VB14-1 after Cr challenge. TCRAV and TCRBV repertoires were analyzed from RNA samples from footpads of Cr-induced ACD mice obtained at day 1, 3 and 7. The frequencies of VA11-1, VA14-1, VB8-2, and VB 14-1 in the Cr-induced ACD mice were significantly increased at days 1, 3, and 7 compared with control mice (*p<0.05, unpaired Student t-test). All experiments were performed in triplicate.