Skip to main content
. 2014 Jan 20;9(1):e85890. doi: 10.1371/journal.pone.0085890

Figure 4. Purification of FGF19 protein from TRXtFGF19 after TEV protease digestion.

Figure 4

(A) Elution profile of Ni-NTA IMAC chromatography. FGF19 Protein didn’t exist in flow-through (FT) fraction as expected, but bound to the column and competitively eluted by increased imidazole concentration. (B) SDS-PAGE analysis the TRXtFGF19 cleavage and tFGF19 purification by Ni-NTA column. Lane 1: purified TRXtFGF19 protein; Lane 2: TRXtFGF19 digested by TEV protease; Lane 3: Flow-through from Ni-NTA column; Lane 4: eluate from Ni-NTA column using 200 mM imidazole; Lane 5: purified TEV protease as control.