Table 1. Analysis of the EDC cross-linked trimers of PfuMoaB-WT and the PfuMoaB-H3 variant by peptide mass fingerprinting.
| Cross-link type | Cross-linked peptides2 | Cross-linked residues2 | N° PSMs1 | |
| WT | H3 | |||
| intra-subunit | SYEEVGYATVLTR - AKSYEEVGYATVLTR | E114-K111 | 6 | 6 |
| SGPLIIEELSK - TGLEIIKSEVFHILK | E41-K157 | 7 | 9 | |
| TGLEIIK - AKSYEEVGYATVLTR | E154-K111 | 3 | 2 | |
| TFKFGVITVSDK - LGEHVYYK | K15-E47 | 4 | 0 | |
| DITIESIKPLFDK - AKSYEEVGYATVLTR | E92-R124 | 0 | 8 | |
| DITIESIKPLFDK - SYEEVGYATVLTR | E92-R124 | 0 | 3 | |
| DITIESIKPLFDKELSFGEVFR - SYEEVGYATVLTR | E92-R124 | 0 | 2 | |
| inter-subunit | DITIESIKPLFDK - DITIESIKPLFDK | E92-K100 | 0 | 6 |
1
trypsinized cross-linked peptides were identified by LC-MS/MS; their sequences and number of peptide-spectrum matches (PSMs) are shown.
2
residues, which are suggested to be cross-linked by EDC are shown in a separate column and underlined in the respective peptide sequences.