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. 2014 Jan 20;9(1):e86145. doi: 10.1371/journal.pone.0086145

Figure 2. The Comparison of Wt-Oligos and Mut-Oligos in the ability of transcription factors combination.

Figure 2

Line 1, the blank control without the nuclear extracts from HeLa cell; Line 2, the Biotin Wt-probe were incubated with the nuclear extracts from HeLa cell; Line 3–4, competition was also performed using Cold Wt Oligos and Cold Mt Oligos; Line 5, the negative control of supershift line, incubated with IgG; Line 6–7, performed with antibodies specific for c-fos, c-jun; Line 8–10, the Mut-probe were showed for the control. These results showed a different allelic binding capacity of AP-1 in rs3783903 of the ITPK1, which is affected by an A→G exchange.