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Light intensity is controlled using a white light LED array and culture temperature is controlled with a Peltier controlled temperature jacket. The PBR is comprised of a magnetically-stirred chamber, filled with a 10% v/v N. oculata stock culture inoculum in f/2 medium. Aeration with ambient air is supplied through a gas dispersion tube. Dissolved oxygen (pO2) is measured optically (PyroScience) and pH is measured electrochemically (Phenometrics).
