Figure 5. Respiratory burst responses of goldfish and lamprey hematopoietic leukocytes following zymosan and apoptotic cell stimulation.

Goldfish PKL and lamprey PTL were incubated with both zymosan and apoptotic cells (5∶1 ratio for each) for 2 h and 6 h, respectively. To examine the effects of a dose response, zymosan and apoptotic cells were added at varying concentrations. Respiratory burst (measured as % DHR positive) was then analyzed for the entire leukocyte population. For all n = 6, examined over a minimum of two independent experiments. Grey dashed line represents the respiratory burst of phagocytes internalizing only AC cells. * p<0.05 and ** p<0.01 compared to grey dashed line (% DHR of phagocytes internalizing only apoptotic cells).