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. 2014 Jan 20;9(1):e86140. doi: 10.1371/journal.pone.0086140

Figure 3. C/EBPβ is required for the formation of long-range, inter-genic interactions between the PPARγ2 promoter and the indicated promoters.

Figure 3

Cells exposed to control shRNA or shRNA targeting C/EBPβ were assayed at 0 h and 6 h post-differentiation. Interaction frequencies between the PPARγ2 promoter and (A) the adiponectin promoter, (B) the leptin promoter, (C) the perilipin 1 promoter, (D) the perilipin 2/ADRP promoter, or (E) the EF1α promoter are presented relative to the intragenic interaction frequency observed between different regions of the TFIIH locus, which served as a normalization control. The interaction frequency at 0 h in the control shRNA treated cells was set to 1; the interaction frequencies for the other samples are presented relative to that value. Results indicate the average of three independent experiments +/− standard deviation. Values for the 6 h control shRNA treated samples were compared to values for the samples treated with C/EBPβ shRNA by one-tailed t-test. ***p<0.001. (F) Western blots indicating C/EBPβ protein levels in control and C/EBPβ shRNA treated cells. PI-3 kinase levels were monitored as a loading control.