Figure 4. Elevation of cAMP levels is required for the formation of long-range, inter-genic interactions between the PPARγ2 promoter and the indicated promoters.

The EF1α promoter is shown as a control. 3C assays were performed on 3T3-L1 cells at 0 or at 6 h post-differentiation in the presence of complete differentiation cocktail or in cocktail lacking the indicated component. Interaction frequencies were quantified as described in the legends for Figures 1–2. Results indicate the average of three independent experiments +/− standard deviation. Values for the 6 h samples differentiated in complete cocktail were compared to values for the samples differentiated in cocktail lacking IBMX by one-tailed t-test. p values are indicated on the graph. ins; insulin. dex; dexamethasone.