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. 2014 Jan 20;9(1):e86243. doi: 10.1371/journal.pone.0086243

Table 2. Primers used for amplifying segments of the MC58 lbpB gene.

Oligo Sequence Description
O3319 GCGGATCCCCAAGGCGGAATATTGCTTC Forward primer with BamH1 site preceding aa 54 of mature LbpB
O2774 CTTACTCTAGACTCATTTTTCCACCTCCTGCATATC Reverse primer with Xba site after stop codon of mature LbpB
O3131 TCAGACGGCATCCTGCCC Forward primer to region encoding aa 534–539 (−LG)
O3130 CGCCTTCGGTTTGGCGGC Reverse primer to region encoding aa 463–468 (−LG)
O3129 TTCGGCGTGGTATTCGGTG Forward primer to region encoding aa 722–727 (−SM)
O3128 AGTTATACCAAGAGATTTCCCATC Reverse primer to to region encoding aa 682–690 (−SM)