Figure 3. Cloned cDNA of A. aegypti glycoprotein hormone leucine-rich repeat containing receptor (AedaeLGR1) and deduced amino acid sequence (GenBank accession number: KF711859).

Exon boundaries were determined by comparing the cloned cDNA against the A. aegypti genome and are denoted by downward pointing arrows. Novel nucleotide and corresponding amino acid sequence arising from unpredicted exons or incorrectly predicted introns in initial transcript prediction (GenBank accession EAT4421) are presented in bold blue font. Further, novel amino acid and nucleotide sequence differing from a second automated transcript prediction (GenBank accession XP_001649032) are indicated by a double underline. Predicted N-linked glycosylation sites are provided in white font outlined by a black box (Asn39, Asn79 and Asn102). Conserved cysteine residues located in the C-terminus of the ectodomain are highlighted by red outlined boxes (Cys₃₅₆, Cys₃₅₇, Cys₃₈₄, Cys₅₂₇, Cys₅₃₉ and Cys₅₄₉). Amino acid sequences forming the predicted seven hydrophobic membrane-spanning domains are denoted in bold red underlined font.