Table 1. Primers designed for initial cloning of cDNA of glycoprotein hormone subunits and its leucine-rich repeat-containing GPCR.

Oligonucleotide name:	Oligonucleotide sequence:	Target and usage:
Aedes GPA2 F1	ATGGAGTGGCTACGATTTGC	sense primer for amplification of A. aegypti GPA2 predicted cDNA
Aedes GPA2 R1	TCAGTCCTTTTTACAGTGGTAACAC	anti-sense primer for amplification of A. aegypti GPA2 predicted cDNA
Aedes GPA2 kozak	GCCACCATGGAGTGGCTACGATTTGC	sense primer introducing Kozak sequence into A. aegypti GPA2 predicted cDNA
Aedes GPA2 HIS-tag	TCAATGGTGATGGTGATGATGGTCCTTTTTACAGTGGT	anti-sense primer for incorporation of hexa histidine-tag on carboxyl terminus of GPA2
Aedes GPB5 F1	ATGATCAATCTAATCTCGGTATGGA	sense primer for amplification of A. aegypti GPB5 predicted cDNA
Aedes GPB5 R1	CTAAGCGTATTCGGATTCGATTT	anti-sense primer for amplification of A. aegypti GPB5 predicted cDNA
Aedes GPB5 kozak	GCCACCATGATCAATCTAATCTCGGT	sense primer introducing Kozak sequence into A. aegypti GPB5 predicted cDNA
Aedes GPB5 HIS-tag	TCAATGGTGATGGTGATGATGAGCGTATTCGGATTCGA	anti-sense primer for incorporation of hexa histidine-tag on carboxyl terminus of GPB5
Aedes LGR1 F1	ATGAGCGTATGTAGTGCACGG	sense primer for amplification of A. aegypti LGR1 predicted cDNA
Aedes LGR1 R1	TTACAGAAAAGTTTCACTTTCCTGC	anti-sense primer for cDNA synthesis andamplification of A. aegypti LGR1 predicted DNA