Table 1. Human Papillomavirus assays compared in the Horizon study.

Human papillomavirus assay	Targeted human papillomavirusgenotypes	Detection technology	DNA/RNA, and the targeted HPV gene	Controls	Certificates
APTIMA	13 HR + 1 LR	MMLV reverse transcriptaseand T7 RNA polymerasemediated amplification	RNA, E6/E7	Spiked control for sample processvalidity	FDA, IVD, CE
CLART	13 HR + 22 LR	Polymerase chain reaction/microarray	DNA, L1	Sample by sample hCFTR gene forsample sufficiency	IVD, CE
Cobas	13 HR + 1 LR	Real-time polymerase chain reaction	DNA, L1	Sample by sampleβ-globin for samplesufficiency	FDA, IVD, CE
Hybrid Capture 2	13 HR	Sandwich capture molecular	DNA, whole genome	Batch control for process	FDA, IVD, CE

Abbreviations: CE = Conformité Européenne mark; FDA = US Food and Drug Administration; HR = high risk genotypes using the International Agency for Research on Cancer’s classification, including the “probably carcinogenic” genotype 68 [26]; IVD = in vitro diagnostic medical device; LR = low risk genotypes using the International Agency for Research on Cancer’s classification, including the “possibly carcinogenic” genotype 66 [26].