Figure 3.

Morpholino efficacy. (A and B) Injection of nephrinMOex25 and podocinMOex3 resulted in the mis-splicing of mRNA as detected by an altered RT-PCR product (arrowheads). Arrows indicate intact mRNA. (A) The efficacy for nephrinMOex25 is consistent from 1 to 4 dpf. (B) By contrast, the efficacy for podocinMOex3 is recognized from 1 to 4 dpf, although a significant expression of intact podocin mRNA is also detected. (C) In the nephrinMOex25 morphants, the protein expression of Nephrin was reduced at 4 dpf larvae. (D) In the podocinMOex3 morphants, a reduced expression of Podocin protein is observed at 3 dpf, however, the expression is recovered at the same level of control at 4 dpf. dpf, days post-fertilization.