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. 2013 Dec 6;9(2):457–465. doi: 10.3892/mmr.2013.1844

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Copyright © 2014, Spandidos Publications

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(A) Co-immunoprecipitation assay for Myc-Nephrin and Flag-Podocin. Following anti-Flag IP, coprecipitated Nephrin was revealed by anti-Myc immuno-blotting (see arrow). Asterisk marks a non-specific protein band. IgH, immunoglobulin heavy chain. (B) Similar input levels of Myc-Nephrin. (C) Expression level of wild-type and Flag-tagged Podocin is notably higher in comparison with that of the R150Q mutant. Myc-Nephrin, Myc-tagged zebrafish Nephrin intracelluar region; Flag-Podocin, Flag-tagged zebrafish full-length Podocin; IP, immunoprecipitation.