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. 2013 Nov 28;9(2):443–449. doi: 10.3892/mmr.2013.1834

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Copyright © 2014, Spandidos Publications

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Effect of VPA on the histone H3 acetylation at the promoter of VEGF. Chromatin immunoprecipitation assay was performed using (A) antibodies against RNA polymerase as a positive control, (B) antibodies against acetylated histone H3 and (C) non-specific mouse IgG as a negative control. qPCR was performed with five pairs of primers specific for the VEGF promoter (P1, P2, P3, I1 and E3). −, control group; +, VPA-treated group; VPA, valproic acid; VEGF, vascular endothelium growth factor; qPCR, quantitative PCR.