Figure 5. Internalization of Alexa-647 labeled antibodies occurred only with VDZ and not with mouse immunoglobulin (mIgG), indicating the internalization is specific for VDZ. Cells were incubated with either Alexa-647 labeled mIgG control or VDZ for 24 h at 37 °C. Cells were gated initially on forward scatter (FCS)/SSC followed by CD4 and CD45RO to obtain the CD4⁺ CD45RO⁺ memory T cells.