Figure 6. Deconvoluted mass spectrum of CHO-produced half-antibodies. (A) The deconvoluted mass spectrum of hole half-antibody shows a glycosylation pattern similar to a normal antibody. (B) The deconvoluted spectrum of knob half-antibody shows a more complicated glycosylation profile, dissimilar from hole shown in (A) and from a regular antibody. (C) The knob antibody was treated with neuraminidase to assess for the presence of sialic acid. The treated antibody shows a reduced complexity that is similar to the hole shown in (A).