Figure 6. C4D2-BsAb inhibited HBsAg release from PLC/PRF/5 cells in vitro. (A) Quantitation of HBsAg levels in the supernatants of PLC/PRF/5 cells cultured with C4G4, D2H2, C4G4 plus D2H2, C4D2-BsAb and control human IgG during the second 24 h. (B) western blotting analysis of cytoplasmic extracts from PLC/PRF/5 cells cultured with these antibodies at the first 24 h. Results were shown as mean ± SD of three independent experiments. *P < 0.05; ^#P > 0.05.