Figure 1. Characterization of GST-VEGF-D. (A) western blot analysis of GST-VEGF-D expression in E. coli. (B) In vitro interaction of GST-VEGF-D and VEGFR-3/Fc. VEGFR3/Fc or VEGF-D proteins were added to 96-well microtiter plates coated with GST-VEGF-D and incubated at 37 °C for 1 h, respectively. The plates were washed for three times and the standard ELISA protocol was followed to detect the binding activity of GST-VEGF-D to VEGFR3/Fc. Results were shown as the means ± SEM of three wells.