Figure 2. Screening and characterization of anti-VEGFR-3 monoclonal antibodies. (A) Inhibition of VEGFR-3/Fc binding to GST-VEGF-D by the mAbs. BBE022 and BDD073 had the inhibitory activities on VEGFR-3/Fc and GST-VEGF-D interaction. Results are shown as the means ± SEM of three wells. (B) Neutralizing activities of BDD073 to the binding activity of VEGFR-3 to GST-VEGF-D in a dose dependent manner. Various antibodies were mixed with 50 ng of VEGFR3/Fc, incubated at 37 °C for 1 h and transferred to 96-well microtiter plates coated with GST-VEGF-D, after an additional 1 h, the plate was washed three times and the standard ELISA protocol was followed to detect the bounded VEGFR3/Fc molecules. BAD045 antibody was used as control. Results are shown as the means ± SEM of three wells.