Table 5. Extent of exchange, recovery and aggregation, and residual reductant following cFAE.
Condition | % by CIEX | Total Protein by A280 (g/L) | % by HP-SEC | Residual 2-MEA (µM) |
||||
---|---|---|---|---|---|---|---|---|
IgG1-F405L-EGFR | bsIgG1-EGFRxCD20 | IgG1-K409R-CD20 | Mon | Agg | Deg | |||
20 g/L; 5 mL |
3.5 | 95.0 | 1.6 | ND | 99.4 | 0.6 | < 0.1 | 20.5 ± 1.2 |
20 g/L; 25 L |
2.8 | 96.3 | 0.9 | 20.0 | 99.4 | 0.6 | < 0.1 | 2.9 ± 0.6 |
ND, not determined; Mon, monomer; Agg, aggregate; Deg, degradation