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. 2013 Aug 22;5(6):962–973. doi: 10.4161/mabs.26233

Table 5. Extent of exchange, recovery and aggregation, and residual reductant following cFAE.

Condition % by CIEX Total Protein by A280 (g/L) % by HP-SEC Residual 2-MEA
(µM)
IgG1-F405L-EGFR bsIgG1-EGFRxCD20 IgG1-K409R-CD20 Mon Agg Deg
20 g/L;
5 mL
3.5 95.0 1.6 ND 99.4 0.6 < 0.1 20.5 ± 1.2
20 g/L;
25 L
2.8 96.3 0.9 20.0 99.4 0.6 < 0.1 2.9 ± 0.6

ND, not determined; Mon, monomer; Agg, aggregate; Deg, degradation