Fig. 4.

Effect of phytosterols of black bean seed coats in the expression of CPT1 (rate-limiting enzyme for β-oxidation of long-chain fatty acids in the liver). It is presented the a relative expression and b protein expression of CPT1, which mediates the transport of long-chain fatty acids across the mitochondrial membrane by binding them to carnitine for β-oxidation, in primary hepatocytes treated with CN = no stimulus, T = the synthetic LXR agonist T0901317, Ph = extract rich in phytosterols associated with black bean seed coat at a dose of 76.13 μg/L. T + Ph = T0901317 and the extract rich in phytosterols associated with black bean seed coat at the same dose (76.13 μg/L). Every experiment was performed in triplicate (n = 3). Data are presented in mean ± SE. Bars with different letter indicate statistically significant difference, P < 0.05