Table 2.
Concentrations of phytosterols in extracts of black bean (mg/100 g of seed coats) before and after acid hydrolysis
| Extract | Stigmasterol | Campesterol | β-Sitosterol | Δ5-Avenasterol | Total | ASG/SG ratio |
|---|---|---|---|---|---|---|
| mg/100 g seed coatsa | ||||||
| Non-hydc | ||||||
| Free | 3.21 ± 0.13 | 10.20 ± 1.54 | 29.42 ± 0.51 | 1.09 ± 0.41 | 43.92 ± 2.50 | |
| ASG | n.d | 23.60 ± 0.85 | 41.23 ± 0.11 | n.d. | 64.83 ± 1.01 | 1.74 |
| SG | n.d. | 32.91 ± 1.21 | n.d. | 4.41 ± 0.08 | 37.32 ± 1.20 | |
| Total | 3.21 ± 0.13 | 66.71 ± 1.99 | 70.65 ± 1.51 | 5.50 ± 0.51 | 146.07 ± 3.54 | |
| Hyd | ||||||
| Free | 9.02 ± 1.12 | 21.17 ± 1.10 | 51.21 ± 1.18 | 2.95 ± 0.94 | 84.35 ± 1.62 | |
ASG acylated steryl glycosides, SG steryl glycosides, Hyd hydrolyzed extract, n.d. not detectable
aMean ± SE of at least three replicates, the conjugated forms are quantified as equivalent of the free forms