Fig. 2.

Osteogenic differentiation. a Expression of runx2, alp and oc osteogenic genes in MSC induced to differentiate into osteoblasts in osteogenic medium alone (OM), or supplemented with either 20 μM of arachidonic acid (AA), 20 μM of AA plus 20 μM of DHA (AA + DHA) or 20 μM of AA plus 20 μM of EPA (AA + EPA), at days 7 and 21 of osteogenic differentiation. The values are relative to OM control (value of 1). b Optical micrographs of MSC induced to differentiate into osteoblasts at day 21, showing the presence of adipocytes when culture medium was supplemented with AA, AA + DHA or AA + EPA. c Quantification of adipocyte number for the different treatments at day 21 of osteogenic induction. *p < 0.05 in relation to OM; #p < 0.05 in relation to AA